Department of Chemical Biology
Yang's Lab,Department of Chemical Biology, Xiamen University
Professor Yang
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Professor
Department of Chemical Biology, Xiamen University
Contact Information
Yang's LaboratoryRoom 532, Lujiaxi Building, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China
Ph: +86 (0) 592-218 7601cyyang@xmu.edu.cn
Huimin Zhang's work has been accepted by Lab Chip and reported by Bytedance
2018-08-20 03:20:55
Fetal aneuploidy and other chromosomal aberrations affect 9 of 1000 live births. Unlike the invasive diagnosis with high risk of miscarriage, noninvasive prenatal diagnosis (NIPD) sampling from maternal blood becomes a promising way for fetal genetic screening. However, fetal cell-based NIPD has a major challenge due to the rare number of fetal cells present in the maternal blood. We designed a Frequency-Enhanced Transferrin receptor Antibody-Labelled Microfluidic Chip (FETAL-Chip) for efficient enrichment and identification of circulating fetal cells, i.e., circulating nucleated red blood cells (cNRBCs) from maternal blood. The FETAL-Chip can dramatically enhance the interaction of fetal cells with antibody-coated micro-posts to increase the capture efficiency while minimizing nonspecific adsorption. With the help of immunostaining, we can identify cNRBCs from as low as 2 milliliter maternal blood. Various numbers of cNRBCs were detected from volunteers as early as 7 weeks after conception and throughout the entire pregnancy. Gene analysis was also carried out to confirm the fetal origin of captured cells. With easy, noninvasive and highly efficient enrichment of cNRBCs, the method presented here offers great potential for noninvasive prenatal diagnosis.
This work has been reported by Bytedance！
