Here, a fully integrated multicolor immunosensor was developed for sensitive and reliable semi-quantitative analysis of HIV-1 p24, which integrates the multistep reactions of horseradish peroxidase (HRP)-linked immunoassay and gold nanorod (AuNR)-based multicolor assay into a single microfluidic chip.The HRP-linked immunoassay is functioned by moving magnetic beads bound to a capture antibody through different aqueous phases containing immunoassay reagents. HRP catalyzed oxidation of 3,3’,5,5’-TetraMethyl benzidine (TMB) is used to mediate AuNRs etching for producing various color changes. Multiple etching processes can be activated by simple mixing of the reagents from reagent storage reservoir. The fully integrated strategy with sample-in-answer-out capability is initiatedby simple chip manipulationand finally concluded by converting a recognition of antigen-antibody into a vivid color variation for direct visualization and semi-quantitative analysis. By bare eye observation, our integrated multicolor immunosensor allows sensitive and reliable semi-quantitative analysis of HIV-1 p24 within 1 hr. The microfluidic chip devicedemonstrated here simplifies the operation significantly and thus allows broader application of multicolor immunosensor for point of care (POC) testing in low-resource settings.