Department of Chemical Biology
Yang's Lab,Department of Chemical Biology, Xiamen University
Home > News > Bingqian Lin's Paper has been Accepted by ACS Applied Materials & Interfaces

Professor Yang

Know more>> Professor Department of Chemical Biology, Xiamen University

Contact Information

Yang's Laboratory

Room 532, Lujiaxi Building, College of Chemistry and Chemical Engineering, Xiamen University, Xiamen 361005, China

Ph: +86 (0) 592-218 7601

Bingqian Lin's Paper has been Accepted by ACS Applied Materials & Interfaces

2016-02-28 11:37:04

     There is considerable demand for sensitive, selective and portable detection of disease-associated proteins, particularly in clinical practice and diagnostic applications. Portable devices are highly desired for detection of disease biomarkers in daily life due to the advantages of being simple, rapid, user-friendly and low-cost. Herein we report an enzyme-encapsulated liposome-linked immunosorbent assay for sensitive detection of proteins using personal glucose meters (PGM) for portable quantitative readout. Liposomes encapsulating a large amount of amyloglucosidase or invertase are surface-coated with recognition elements such as aptamers or antibodies for target recognition. By translating molecular recognition signal into a large amount of glucose with the encapsulated enzyme, disease biomarkers such as thrombin or C-reactive protein (CRP) can be quantitatively detected by a PGM with high a detection limit of 1.8 nM or 0.30 nM, respectively. With the advantages of portability, ease of use, and low-cost, the method reported here has potential for portable and quantitative detection of various targets for different POC testing scenarios, such as rapid diagnosis in clinic offices, health monitoring at the bedside, and chemical/biochemical safety control in the field.